Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

Food Science and Technology (Campinas)

Cintia Anabela Mazzucotelli¹  Alejandra Graciela Ponce¹  Catalina Elena Kotlar¹  María Del Rosario Moreira¹ 

[1] ,Universidad Nacional de Mar del Plata Grupo de Investigación en Ingeniería en Alimentos Mar del Plata,Argentina

关键词: hydrolytic profile;    waste management;    bioconversion;   

DOI  :  10.1590/S0101-20612013005000038

来源: SciELO

【 摘 要 】

There is a trend towards the use of novel technologies nowadays, mainly focused on biological processes, for recycling and the efficient utilization of organic residues that can be metabolized by different microorganisms as a source of energy. In the present study the isolation of bacterial strains from six different agro-industrial by-products and waste was performed with the objective of evaluating their hydrolytic capacities and suitability for use in bioconversion of specific substrates. The 34 isolated strains were screened in specific culture media for the production of various hydrolytic enzymes (lipase, protease, cellulase, and amylase). It was found that 28 strains exhibited proteolytic activity, 18 had lipolytic activity, 13 had caseinolytic activity, 15 had amylolytic activity, and 11 strains exhibited cellulolytic activity. The strains that showed the highest hydrolytic capacities with biotechnological potential were selected, characterized genotipically, and identified as Bacillus, Serratia, Enterococcus, Klebsiella, Stenotrophomonas, Lactococcus, and Escherichia genera. It was concluded that the strain isolates have a high potential for use in the bioconversion of agro-industrial waste, both as a pure culture and as a microbial consortium.

【 授权许可】

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