Application of Doehlert experimental design in the optimization of experimental variables for the Pseudozyma sp. (CCMB 306) and Pseudozyma sp. (CCMB 300) cell lysis

Food Science and Technology (Campinas)

Amanda Reges De Sena¹  Gildomar Lima Valasques Júnior¹  Ingara Keisle São Paulo Barretto¹  Sandra Aparecida Assis¹ 

[1] ,Universidade Estadual de Feira de Santana - UEFS Departamento de Saúde Feira de Santana BA ,Brasil

关键词: lytic enzymes;    multivariate optimization;    doehlert matrix design;    enzimas líticas;    otimização multivariada;    matriz doehlert;   

DOI  :  10.1590/S0101-20612012005000118

来源: SciELO

【 摘 要 】

This study aimed to verify the influence of pH and temperature on the lysis of yeast using experimental design. In this study, the enzymatic extract containing β-1,3-glucanase and chitinase, obtained from the micro-organism Moniliophthora perniciosa, was used. The experiment showed that the best conditions for lysis of Pseudozyma sp. (CCMB 306) and Pseudozyma sp. (CCMB 300) by lytic enzyme were pH 4.9 at 37 ºC and pH 3.9 at 26.7 ºC, respectively. The lytic enzyme may be used for obtaining various biotechnology products from yeast.

【 授权许可】

CC BY   
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