Elongated cells of Listeria monocytogenes in biofilms in the presence of sucrose and bacteriocin-producing Leuconostoc mesenteroides A11

Food Science and Technology (Campinas)

Regiane Priscilla Ratti¹  Bruna Carrer Gomes¹  Rafael Chacon Ruiz Martinez¹  Vanessa Maciel Souza¹  Elaine Cristina Pereira De Martinis¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Análises Clínicas, Toxicológicas e BromatológicasRibeirão Preto SP ,Brasil

关键词: L. monocytogenes;    biofilm;    elongated cells;    lactic acid bacteria;    bacteriocins;    L. monocytogenes;    biofilme;    células alongadas;    bactérias láticas;    bacteriocinas;   

DOI  :  10.1590/S0101-20612010000400027

来源: SciELO

【 摘 要 】

Listeria monocytogenes is a foodborne pathogen which may survive in biofilms and persist in food processing plants. In this study, the ability of Leuconostoc mesenteroides (bac+ and bac-) to inhibit biofilm formation by L. monocytogenes ATCC 19115 was studied with stainless steel coupons immersed in BHI broth and BHI broth plus sucrose in combination with the Lactic Acid Bacteria (LAB). Adhered cells were collected with swabs and enumerated on selective agars (Oxford for listeria and MRS for leuconostoc). Leuconostoc mesenteroides bac+ in co-culture with L. monocytogenes was effective to inhibit biofilm formation by listeria for up to 3 hours of incubation, but at 24 hours, biofilm was present in all conditions tested, as confirmed by observations of stainless steel coupons under Scanning Electron Microscopy (SEM). It was also observed that in the presence of L. mesenteroides bac+ in BHI plus sucrose, a high number of elongated cells of L. monocytogenes was present, which may indicate an adaptation response of the pathogen to stress conditions with important implications for food safety.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License