Head Space Solid Phase Micro-Extraction (HS - SPME) of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636)

Food Science and Technology (Campinas)

Eunice Valduga¹  Alexsandra Valerio¹  Helen Treichel¹  Irajá Nascimento Filho²  Agenor Fúrigo Júnior²  Marco Di Luccio¹ 

[1] ,Universidade Regional Integrada do Alto Uruguai e das Missões Departamento de Engenharia de Alimentos Erechim RS ,Brasil

关键词: bio-production;    Sporidiobolus salmonicolor;    SPME;    aroma;    experimental design;    bioprodução;    Sporidiobolus salmonicolor;    SPME;    aroma;    planejamento de experimentos;   

DOI  :  10.1590/S0101-20612010000400023

来源: SciELO

【 摘 要 】

The aim of the present study was the assessment of volatile organic compounds produced by Sporidiobolus salmonicolor (CBS 2636) using methyl and ethyl ricinoleate, ricinoleic acid and castor oil as precursors. The analysis of the volatile organic compounds was carried out using Head Space Solid Phase Micro-Extraction (HS - SPME). Factorial experimental design was used for investigating extraction conditions, verifying stirring rate (0-400 rpm), temperature (25-60 ºC), extraction time (10-30 minutes), and sample volume (2-3 mL). The identification of volatile organic compounds was carried out by Gas Chromatography with Mass Spectrum Detector (GC/MSD). The conditions that resulted in maximum extraction were: 60 ºC, 10 minutes extraction, no stirring, sample volume of 2.0 mL, and addition of saturated KCl (1:10 v/v). In the bio-production of volatile organic compounds the effect of stirring rate (120-200 rpm), temperature (23-33 ºC), pH (4.0-8.0), precursor concentration (0.02-0.1%), mannitol (0-6%), and asparagine concentration (0-0.2%) was investigated. The bio-production at 28 ºC, 160 rpm, pH 6,0 and with the addition of 0.02% ricinoleic acid to the medium yielded the highest production of VOCs, identified as 1,4-butanediol, 1,2,2-trimethylciclopropilamine, beta-ionone; 2,3-butanodione, pentanal, tetradecane, 2-isononenal, 4-octen-3-one, propanoic acid, and octadecane.

【 授权许可】

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