期刊论文详细信息
Brazilian Journal of Medical and Biological Research
Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model
N.q. Li1  J. Yang1  L. Cui1  N. Ma1  L. Zhang1  L.r. Hao1 
关键词: microRNA;    miR-483;    Renal fibrosis;    Igf2;    UUO;   
DOI  :  10.1590/1414-431X20143958
来源: SciELO
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【 摘 要 】

The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgfexpression, as determined by Pearson's correlation analysis. Hgf expression significantly increased at 1 and 2 weeks after the surgery compared to the control group. This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model. These data suggest that miR-483 and miR-483* play a role in renal fibrosis and that miR-483* may interact with miR-483 in renal fibrosis. Thus, these miRNAs may play a role in the pathogenesis of renal fibrosis and coexpression of their host gene Igf2.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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