Pattern of Wnt ligand expression during chick eye development

Brazilian Journal of Medical and Biological Research

E. Rossi¹  F. Siwiec¹  C.y.i. Yan¹ 

[1] ,Universidade de São Paulo Instituto de Ciências Biomédicas Departamento de Biologia Celular e do DesenvolvimentoSão Paulo SP ,Brasil

关键词: Wnt-5a;    Optic vesicle;    Optic development;    Wnt;    Anterior neural tube;   

DOI  :  10.1590/S0100-879X2006005000155

来源: SciELO

【 摘 要 】

The dorsoventral axis of the eye is determined prior to optic cup invagination. A variety of signaling pathways have been implicated in the maintenance of the optic dorsoventral axis, including, but not limited to, bone morphogenetic protein 4, Sonic Hedgehog and retinoic acid. Here, we investigated the possible contribution of Wnt ligands to the establishment or maintenance of the optic axis by analyzing their expression pattern during early chick optic development. We performed in situ hybridization of Wnt-1, Wnt-3a, Wnt-4, and Wnt-5a during the optic vesicle, early optic cup and established optic cup stages and focused our analysis on the optic region. Our data showed that Wnt-5a, but none of the others, is expressed in the dorsal region of the eye starting from the Hamburger and Hamilton stage 14 (HH14). These results are supported by cryosections of the labeled optic region, which further reveal that Wnt-5a is expressed only in the dorsal retinal pigmented epithelium. Thus, we propose that Wnt-5a is a marker for dorsal retinal pigmented epithelium in chick embryos from HH14 to HH19.

【 授权许可】

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