Lipoprotein lipase PvuII polymorphism is associated with variations in serum lipid levels in non-diabetic pregnant women

Brazilian Journal of Medical and Biological Research

R.j.c. Sepetiba²  J. Andrade¹  R.d.c. Hirata¹  M.h. Hirata¹  C.r.g. Sepetiba¹  Y. Nakamura¹  L.o. Matsumoto¹  S.a. Cavalli¹  M.c. Bertolami¹ 

[1],Universidade de São Paulo Faculdade de Medicina Instituto do Coração

关键词: Lipoprotein lipase;    LPL PvuII polymorphism;    Apolipoprotein E;    Single nucleotide polymorphism;    Serum lipids;    Non-diabetic pregnancy;   

DOI  :  10.1590/S0100-879X2006005000102

来源: SciELO

【 摘 要 】

The aim of the present study was to determine if there is an association between the single nucleotide polymorphisms (SNPs) of the lipoprotein lipase (LPL) and apolipoprotein E (apo E) genes and the serum lipid profile in pregnancy and puerperium. Non-diabetic women of European descent in the third semester of pregnancy (N = 120) were selected. Those with diseases or other condition that could modify their lipid profile were excluded from the study (N = 32). Serum lipids were measured by routine laboratory procedures and genomic DNA was extracted by a salting out method. LPL (PvuII and HindIII) and apo E (HhaI) SNPs were detected by the polymerase chain reaction and restriction fragment length polymorphism. Categorical and continuous variables were compared by the chi-square test and Student t-test or ANOVA, respectively. Women carrying the LPL P1P1 genotype had higher serum LDL cholesterol (N = 21; 155 ± 45 mg/dL) than women carrying the P1P2/P2P2 genotypes (N = 67; 133 ± 45 mg/dL; P = 0.032). During the puerperium period, serum levels of triglycerides and VLDL cholesterol were significantly reduced in women carrying the P1P1 (73%, P = 0.006) and P1P2 (51%, P = 0.002) genotypes but not in women carrying the P2P2 genotype (23%, P > 0.05). On the other hand, serum concentrations of lipids did not differ between the LPL HindIII and apo E genotypes during pregnancy and after delivery. We conclude that LPL PvuII SNP is associated with variations in serum lipids during pregnancy and the puerperal period in non-diabetic women.

【 授权许可】

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