Brazilian Journal of Medical and Biological Research | |
Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells | |
A.j. Merjan2  C.a. Kanashiro2  J.e. Krieger1  S.w. Han2  A.c.m. Paiva2  | |
[1] ,Universidade Federal de São Paulo Escola Paulista de Medicina Departamento de BiofísicaSão Paulo SP ,Brasil | |
关键词: angiotensin II; agonist-induced receptor internalization; endocytosis; fluorescence-tagged receptor; nuclear angiotensin receptors; renin-angiotensin system; | |
DOI : 10.1590/S0100-879X2001000900011 | |
来源: SciELO | |
【 摘 要 】
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
【 授权许可】
CC BY
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