Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

Brazilian Journal of Medical and Biological Research

A.j. Merjan²  C.a. Kanashiro²  J.e. Krieger¹  S.w. Han²  A.c.m. Paiva² 

[1] ,Universidade Federal de São Paulo Escola Paulista de Medicina Departamento de BiofísicaSão Paulo SP ,Brasil

关键词: angiotensin II;    agonist-induced receptor internalization;    endocytosis;    fluorescence-tagged receptor;    nuclear angiotensin receptors;    renin-angiotensin system;   

DOI  :  10.1590/S0100-879X2001000900011

来源: SciELO

【 摘 要 】

A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.

【 授权许可】

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