Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase

Brazilian Journal of Medical and Biological Research

C.r.c. Franco²  H.a.o. Rocha²  E.s. Trindade²  I.a.n. Santos²  E.l. Leite¹  S.s. Veiga¹  H.b. Nader²  C.p. Dietrich² 

[1] ,Universidade Federal de São Paulo Escola Paulista de Medicina Departamento de BioquímicaSão Paulo SP ,Brasil

关键词: CHO cells;    extracellular matrix proteins;    proteoglycans;    glycosaminoglycans;    cell adhesion;    cell proliferation;   

DOI  :  10.1590/S0100-879X2001000800001

来源: SciELO

【 摘 要 】

We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient in the synthesis of proteoglycans due to lack of activity of xylosyl transferase. Using different amounts of wild-type and mutant cells, little adhesion was observed in the presence of laminin and type I collagen. However, when fibronectin or vitronectin was used as substrate, there was an enhancement in the adhesion of wild-type and mutant cells. Only CHO-K1 cells showed a time-dependent adhesion on type IV collagen. These results suggest that the two cell lines present different adhesive profiles. Several lines of experimental evidence suggest that heparan sulfate proteoglycans play a role in cell adhesion as positive modulators of cell proliferation and as key participants in the process of cell division. Proliferation and cell cycle assays clearly demonstrate that a decrease in the amount of glycosaminoglycans does not inhibit the proliferation of mutant CHO-745 cells when compared to the wild type CHO-K1, in agreement with the findings that both CHO-K1 and CHO-745 cells take 8 h to enter the S phase.

【 授权许可】

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