Rat liver responsiveness to gluconeogenic substrates during insulin-induced hypoglycemia

Brazilian Journal of Medical and Biological Research

H.m. De Souza²  G.r. Borba-murad²  R.b. Ceddia¹  R. Curi¹  M. Vardanega-peicher¹  R.b. Bazotte¹ 

[1] ,Universidade Estadual de Londrina Departamento de Ciências Fisiológicas Londrina PR ,Brasil

关键词: insulin-induced hypoglycemia;    hepatic glucose production;    gluconeogenesis;    alainne;    glutamine;   

DOI  :  10.1590/S0100-879X2001000600012

来源: SciELO

【 摘 要 】

Hepatic responsiveness to gluconeogenic substrates during insulin-induced hypoglycemia was investigated. For this purpose, livers were perfused with a saturating concentration of 2 mM glycerol, 5 mM L-alanine or 5 mM L-glutamine as gluconeogenic substrates. All experiments were performed 1 h after an ip injection of saline (CN group) or 1 IU/kg of insulin (IN group). The IN group showed higher (P<0.05) hepatic glucose production from glycerol, L-alanine and L-glutamine and higher (P<0.05) production of L-lactate, pyruvate and urea from L-alanine and L-glutamine. In addition, ip injection of 100 mg/kg glycerol, L-alanine and L-glutamine promoted glucose recovery. The results indicate that the hepatic capacity to produce glucose from gluconeogenic precursors was increased during insulin-induced hypoglycemia.

【 授权许可】

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