Cytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR

Brazilian Journal of Medical and Biological Research

V.h. Aquino¹  L.t.m. Figueiredo¹ 

[1],Universidade de São Paulo Faculdade de Medicina de Ribeirão Preto Unidade Multidepartamental de Pesquisa em VirologiaRibeirão Preto SP ,Brasil

关键词: cytomegalovirus;    polymerase chain reaction;    kidney transplantation;   

DOI  :  10.1590/S0100-879X2001000100011

来源: SciELO

【 摘 要 】

A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection in blood and urine samples during the post-transplantation period. CMV was detected more frequently in blood samples than in urine samples (P<0.001). Thirty-three patients had CMV detected at least once in blood and/or urine samples. Seven of these patients (21.2%) were diagnosed as having symptomatic CMV infection and showed a worse clinical outcome, with a higher death rate (P = 0.03). No association between CMV viremia and graft rejection was observed. Nested-PCR was not useful to identify patients at risk for symptomatic CMV infection since only 21.2% of the patients with CMV infection were symptomatic.

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