Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion

Brazilian Journal of Medical and Biological Research

N.a. Silveira²  C.r. Franci¹ 

[1] ,Universidade de GoiásGoiânia GO ,Brasil

关键词: neuropeptide Y;    medial preoptic area;    neuropeptide Y-Y1 receptor;    prolactin;    mRNA for neuropeptide;    Y-Y1 receptor;    luteinizing hormone;    follicle-stimulating hormone;   

DOI  :  10.1590/S0100-879X1999000900016

来源: SciELO

【 摘 要 】

We investigated the participation of neuropeptide Y-Y1 receptors within the medial preoptic area in luteinizing hormone, follicle-stimulating hormone and prolactin release. Four bilateral microinjections of sense (control) or antisense 18-base oligonucleotides of messenger ribonucleic acid (mRNA) (250 ng) corresponding to the NH2-terminus of the neuropeptide Y1 receptor were performed at 12-h intervals for two days into the medial preoptic area of ovariectomized Wistar rats (N = 16), weighing 180 to 200 g, treated with estrogen (50 µg) and progesterone (25 mg) two days before the experiments between 8.00 and 10:00 a.m. Blockade of Y1 receptor synthesis in the medial preoptic area by the antisense mRNA did not change plasma luteinizing hormone or follicle-stimulating hormone but did increase prolactin from 19.6 ± 5.9 ng/ml in the sense group to 52.9 ± 9.6 ng/ml in the antisense group. The plasma hormones were measured by radioimmunoassay and the values are reported as mean ± SEM. These data suggest that endogenous neuropeptide Y in the medial preoptic area has an inhibitory action on prolactin secretion through Y1 receptors.

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