Functional analysis of newly discovered growth control genes: experimental approaches

Brazilian Journal of Medical and Biological Research

R.b. Flatschart¹  M.c. Sogayar¹ 

[1] ,Universidade de São PauloSão Paulo SP ,Brasil

关键词: gene function;    transgene overexpression;    expression interference;    antisense;    ribozymes;    transgenic cells and animals;    genetic knock-out;   

DOI  :  10.1590/S0100-879X1999000700011

来源: SciELO

【 摘 要 】

A large number of DNA sequences corresponding to human and animal transcripts have been filed in data banks, as cDNAs or ESTs (expression sequence tags). However, the actual function of their corresponding gene products is still largely unknown. Several of these genes may play a role in regulation of important biological processes such as cell division, differentiation, malignant transformation and oncogenesis. Elucidation of gene function is based on 2 main approaches, namely, overexpression and expression interference, which respectively mimick or suppress a given phenotype. The currently available tools and experimental approaches to gene functional analysis and the most recent advances in mass cDNA screening by functional analysis are discussed.

【 授权许可】

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