Effects of sciatic-conditioned medium on neonatal rat retinal cells in vitro

Brazilian Journal of Medical and Biological Research

P.m.m. Torres¹  C.v.v. Guilarducci¹  E.g. Araujo¹ 

[1] ,Universidade Federal Fluminense

关键词: cytokines;    neurotrophic factors;    retina;    Schwann cells;    sciatic nerve;   

DOI  :  10.1590/S0100-879X1998001100012

来源: SciELO

【 摘 要 】

Schwann cells produce and release trophic factors that induce the regeneration and survival of neurons following lesions in the peripheral nerves. In the present study we examined the in vitro ability of developing rat retinal cells to respond to factors released from fragments of sciatic nerve. Treatment of neonatal rat retinal cells with sciatic-conditioned medium (SCM) for 48 h induced an increase of 92.5 ± 8.8% (N = 7 for each group) in the amount of total protein. SCM increased cell adhesion, neuronal survival and glial cell proliferation as evaluated by morphological criteria. This effect was completely blocked by 2.5 µM chelerythrine chloride, an inhibitor of protein kinase C (PKC). These data indicate that PKC activation is involved in the effect of SCM on retinal cells and demonstrate that fragments of sciatic nerve release trophic factors having a remarkable effect on neonatal rat retinal cells in culture.

【 授权许可】

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