Purification of bovine pancreatic glucagon as a by-product of insulin production

Brazilian Journal of Medical and Biological Research

A.s.r. Andrade²  L. Vilela¹  H. Tunes¹ 

[1] ,Universidade Federal de Minas Gerais

关键词: glucagon;    protein purification;    gel filtration;    ion-exchange chromatography;    insulin;   

DOI  :  10.1590/S0100-879X1997001200006

来源: SciELO

【 摘 要 】

A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation. The isoelectric precipitate containing glucagon was then purified by ion-exchange chromatography on Q-Sepharose FF, gel filtration on Sephadex G-25 and ion-exchange chromatography on S-Sepharose FF. A pilot scale test was performed with a recovery of 87.6% and a purification factor of 8.78 for the first chromatographic step, a recovery of 75.1% and a purification factor of 3.90 for the second, and a recovery of 76.2% and a purification factor of 2.36 for the last one. The overall yield was 50%, a purification factor of 80.8 was obtained and the fraction containing active glucagon (suitable for pharmaceutical preparations) was 84% pure as analyzed by HPLC

【 授权许可】

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