Brazilian Journal of Medical and Biological Research | |
Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain | |
E.a. Miranda1  G. De-murcia1  J. Ménissier-de-murcia1  | |
[1] ,Ecole Supérieure de Biotechnologie de Strasbourg | |
关键词: PARP; insect cells; baculovirus; overproduction; | |
DOI : 10.1590/S0100-879X1997000800002 | |
来源: SciELO | |
【 摘 要 】
A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 109 infected cells in three liters of culture
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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