Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain

Brazilian Journal of Medical and Biological Research

E.a. Miranda¹  G. De-murcia¹  J. Ménissier-de-murcia¹ 

[1] ,Ecole Supérieure de Biotechnologie de Strasbourg

关键词: PARP;    insect cells;    baculovirus;    overproduction;   

DOI  :  10.1590/S0100-879X1997000800002

来源: SciELO

【 摘 要 】

A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 109 infected cells in three liters of culture

【 授权许可】

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