Morphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity

Pesquisa Veterinária Brasileira

Fernanda C. Santos¹  Juneo F. Silva¹  Jankerle N. Boeloni¹  Edgar Teixeira¹  Eduardo M. Turra¹  Rogéria Serakides¹  Natália M. Ocarino¹ 

关键词: Angiogenic factor;    apoptotic factor;    thyroid receptors;    ovary;    tilapia;    Oreochromis niloticus;    fish;    immunohistochemistry.;    Fator angiogênico;    fator apoptótico;    receptores tireoidianos;    ovário;    tilápia;    Oreochromis niloticus;    peixe;    imuno-histoquímica.;   

DOI  :  10.1590/S0100-736X2015000400010

来源: SciELO

【 摘 要 】

Morphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity were studied. The morphological evaluation of the ovaries was performed by histological paraffin embedded and stained with HE. The immunohistochemical expressions of CDC47, VEGF, Flk-1, angiopoietin, Tie-2 and thyroid receptor (TRα) were performed by the technique of streptavidein-biotin-peroxidase. Apoptosis was assessed using the TUNEL kit. The relative expression of thyroid hormone receptors (TRα and TRβ) was assessed by RT-PCR real time. The nuclear expression of CDC47 increased with the stage of maturation of the oocyte and was observed in the follicle cells. Apoptotic bodies were observed in the follicular cells of atretic follicles and postovulatory follicles from the ovaries of 150g and 350g fish. Expression of VEGF and its receptor Flk-1 was also observed in the follicular cells, and the expression of both increased with the maturity of the oocyte, with a higher intensity observed in the full-grown follicle. The expression of angiopoietin and of its receptor (Tie 2) was discrete and moderate respectively. TRα expression was independent of follicular development. However, the 350 g tilapia exhibited higher expression of TRβ compared with the 50 g tilapia. We conclude that the proliferative activity and the expression of VEGF and its receptor increase with follicular maturation and that the TRs expression increases with ovarian maturity in tilapia (Oreochromis niloticus).

【 授权许可】

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