Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine

Pesquisa Veterinária Brasileira

Elena Mettifogo¹  Melissa Buzinhani¹  Marcos R. Buim¹  Jorge Timenetsky¹  Antonio J. Piantino Ferreira¹ 

关键词: Chicken;    Mycoplasma gallisepticum;    Mycoplasma synoviae;    multiplex PCR.;    Galinha;    Mycoplasma gallisepticum;    Mycoplasma synoviae;    PCR multiplex.;   

DOI  :  10.1590/S0100-736X2015000100004

来源: SciELO

【 摘 要 】

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the mycoplasma infections of most concern for commercial poultry industry. MG infection is commonly designated as chronic respiratory disease (CRD) of chickens and infections sinusitis of turkeys. MS causes sub clinical upper respiratory infection and tenosynovitis or bursitis in chickens and turkeys. The multiplex PCR was standardized to detect simultaneously the MS, MG field strains and MG F-vaccine strain specific. The generic PCR for detection of any species of Mollicutes Class was performed and compared to the multiplex PCR and to PCR using species-specific primers. A total of 129 avian tracheal swabs were collected from broiler-breeders, layer hens and broilers in seven different farms and were examined by multiplex PCR methods. The system (multiplex PCR) demonstrated to be very rapid, sensitive, and specific. Therefore, the results showed a high prevalence of MS in the flocks examined (27.9%), and indicate that the MS is a recurrent pathogen in Brazilian commercial poultry flocks.

【 授权许可】

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