Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará

Pesquisa Veterinária Brasileira

Jenevaldo B. Silva²  Cinthia T. A. Lopes¹  Cleyton P. Pinheiro¹  Danilo H.s. Lima¹  Roberto S. L. Silva¹  Adivaldo H. Fonseca¹  Flábio R. Araújo¹  José D. Barbosa-neto¹ 

[1] ,Universidade Estadual Paulista Facudade de Ciências Agrárias e Veterinárias Laboratório de ImunodiagnósticoJaboticabal SP ,Brasil

关键词: Babesia bovis;    Babesia bigemina;    buffaloes;    Bubalus bubalis;    ELISA;    qPCR;    Marajó Island;    Babesia bovis;    Babesia bigemina;    búfalos;    Bubalus bubalis;    ELISA;    qPCR;    Ilha de Marajó;   

DOI  :  10.1590/S0100-736X2013000700003

来源: SciELO

【 摘 要 】

The aim of the study was to estimate the prevalence of Babesia bovis and Babesia bigemina in water buffaloes of the Marajó Island, State of Pará, Brazil. We used an indirect enzyme-linked immunosorbent assay (iELISA), with total antigen containing proteins outer surface, and polymerase chain reaction (qPCR), involving the use of SYBR Green based on amplification of a small fragment of the cytochrome b gene. The prevalence of positive animals in iELISA to B. bovis B. bigemina and mixed infection was 24.87% (199/800), 20.75% (166/800) and 18.75% (150/800), respectively. Using the PCR, the presence of B. bovis was detected in 15% (18/199) and B. bigemina in 16% (19/199) of animals, and of these, 58% (11/19) presented co-infected by the two agents. The results show a low prevalence of antibodies anti-B. bovis and anti-B. bigemina in water buffaloes from Marajó Island. However, it was observed that the agents of bovine babesiosis circulate in buffaloes, and these may act as reservoirs.

【 授权许可】

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