Comparison of two methods of tear sampling for protein quantification by Bradford method

Pesquisa Veterinária Brasileira

Eliana Farias²  Katia L. Yasunaga²  Romulo V.r. Peixoto²  Micaella P. Fonseca¹  Wagner Fontes¹  Paula D. Galera² 

[1] ,Universidade de Brasília Faculdade de Agronomia e Medicina Veterinária Brasília DF ,Brazil

关键词: Tear sampling;    protein;    Schirmer;    microcapillary;    Bradford method;    Lágrima;    proteína;    Schirmer;    microcapillar;    Bradford;   

DOI  :  10.1590/S0100-736X2013000200021

来源: SciELO

【 摘 要 】

The aim of this study was to compare two methods of tear sampling for protein quantification. Tear samples were collected from 29 healthy dogs (58 eyes) using Schirmer tear test (STT) strip and microcapillary tubes. The samples were frozen at -80ºC and analyzed by the Bradford method. Results were analyzed by Student's t test. The average protein concentration and standard deviation from tears collected with microcapillary tube were 4.45mg/mL ±0.35 and 4,52mg/mL ±0.29 for right and left eyes respectively. The average protein concentration and standard deviation from tears collected with Schirmer Tear Test (STT) strip were and 54.5mg/mL ±0.63 and 54.15mg/mL ±0.65 to right and left eyes respectively. Statistically significant differences (p<0.001) were found between the methods. In the conditions in which this study was conducted, the average protein concentration obtained with the Bradford test from tear samples obtained by Schirmer Tear Test (STT) strip showed values higher than those obtained with microcapillary tube. It is important that concentration of tear protein pattern values should be analyzed according the method used to collect tear samples.

【 授权许可】

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