Pesquisa Veterinária Brasileira | |
A phylogenetic study of canine parvovirus type 2c in midwestern Brazil | |
Danúbia S. Fontana2  Paulo Ricardo D. Rocha1  Raquel A.s. Cruz2  Letícya L. Lopes2  Andréia L.t. Melo2  Marcelo M. Silveira2  Daniel M. Aguiar2  Caroline A. Pescador2  | |
[1] ,Universidade Federal de Mato Grosso Faculdade de Agronomia, Medicina Veterinária e Zootecnia Departamento de Clínica Médica VeterináriaCuiabá MT ,Brazil | |
关键词: Canine parvovirus; enteritis; PCR; DNA; sequencing; Parvovirus canino; enterites; PCR; DNA; sequenciamento; | |
DOI : 10.1590/S0100-736X2013000200013 | |
来源: SciELO | |
【 摘 要 】
Since the late 1970s, canine parvovirus type 2 (CPV-2) has emerged as a causative agent of fatal severe acute hemorrhagic enteritis in dogs. To date, three antigenic types of CPV-2 were described worldwide (CPV-2a/b/c). This study was conducted to determine the variants of CPV-2 circulating in dogs from the Cuiabá Municipality in Midwestern Brazil. Out of 50 fecal samples, collected between 2009 and 2011, 27 tested positive for CPV-2. A 583 bp fragment of the VP2 gene was amplified by PCR, 13 representative samples were analyzed further by DNA sequencing. All strains were characterized as CPV-2c, displayed a low genetic variability although observed several amino acid substitution. These findings indicated that CPV-2c has been circulating in dogs from the Cuiabá Municipality in Midwestern Brazil.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
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