Molecular confirmation of ovine herpesvirus 2-induced malignant catarrhal fever lesions in cattle from Rio Grande do Norte, Brazil

Pesquisa Veterinária Brasileira

Selwyn A. Headley²  Isadora K.f. Sousa¹  Antonio H.h. Minervino¹  Isabella O. Barros¹  Raimundo A. Barrêto Júnior¹  Alice F. Alfieri¹  Enrico L. Ortolani¹  Amauri A. Alfieri¹ 

[1] ,Universidade Norte do Paraná Faculdade de Medicina Veterinária Laboratório de Patologia VeterináriaArapongas PR ,Brazil

关键词: Cattle disease;    ovine herpesvirus 2;    malignant catarrhal fever;    necrotizing vasculitis;    gammaherpesvirus;    Doenças de bovinos;    herpes vírus ovino tipo 2;    febre catarral malígna;    vasculite necrosante;    gammaherpesvírus;   

DOI  :  10.1590/S0100-736X2012001200001

来源: SciELO

【 摘 要 】

Molecular findings that confirmed the participation of ovine herpesvirus 2 (OVH-2) in the lesions that were consistent with those observed in malignant catarrhal fever of cattle are described. Three mixed-breed cattle from Rio Grande do Norte state demonstrated clinical manifestations that included mucopurulent nasal discharge, corneal opacity and motor incoordination. Routine necropsy examination demonstrated ulcerations and hemorrhage of the oral cavity, corneal opacity, and lymph node enlargement. Significant histopathological findings included widespread necrotizing vasculitis, non-suppurative meningoencephalitis, lymphocytic interstitial nephritis and hepatitis, and thrombosis. PCR assay performed on DNA extracted from kidney and mesenteric lymph node of one animal amplified a product of 423 base pairs corresponding to a target sequence within the ovine herpesvirus 2 (OVH-2) tegument protein gene. Direct sequencing of the PCR products, from extracted DNA of the kidney and mesenteric lymph node of one cow, amplified the partial nucleotide sequences (423 base pairs) of OVH-2 tegument protein gene. Blast analysis confirmed that these sequences have 98-100% identity with similar OVH-2 sequences deposited in GenBank. Phylogenetic analyses, based on the deduced amino acid sequences, demonstrated that the strain of OVH-2 circulating in ruminants from the Brazilian states of Rio Grande do Norte and Minas Gerais are similar to that identified in other geographical locations. These findings confirmed the active participation of OVH-2 in the classical manifestations of sheep associated malignant catarrhal fever.

【 授权许可】

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