Phylogenetic and pathotype analysis of Escherichia coli swine isolates from Southern Brazil

Pesquisa Veterinária Brasileira

Lilian Kolling Girardini²  Franciele M. Siqueira¹  Carina C. Krewer²  Cristina C. Krewer¹  Mateus M. Da Costa¹  Agueda C. De Vargas² 

[1] ,Universidade Federal de Santa Maria Departamento de Medicina Veterinária Preventiva Santa Maria RS ,Brazil

关键词: Escherichia coli;    E. coli typification;    phylogeny;    uropathogenic E. coli;    enterotoxigenic E. coli;    Escherichia coli;    tipificação de E. coli;    filogenia;    E. coli uropatogênica;    E. coli enterotoxigênica;   

DOI  :  10.1590/S0100-736X2012000500002

来源: SciELO

【 摘 要 】

The current study evaluated the presence of virulence factors by a multiplex PCR technique and then phylogenetically classified the studied strains into groups A, B1, B2 and D, according to Clermont et al. (2000), in 152 intestinal and extraintestinal swine isolates of Escherichia coli. Seventy seven isolates tested were positive for virulence factors. Phylogenetic characterization placed 21 samples into group A, 65 into B1, 19 into B2 and 47 into D. Fourteen urine samples were classified as uropathogenic E. coli (UPEC), nine were both UPEC and enterotoxigenic E. coli (ETEC) and four were ETEC only. The most common phylogenetic classifications were B1 and D groups. Of the analyzed fecal samples, 25 were classified as ETEC. Phylogenetically, the group of higher occurrence was B1, followed by B2, A and D. For the small intestine samples, 20 were classified as ETEC. Phylogenetic analysis found groups B1 and A to be the most commons in these samples. Six isolated tissue samples were classified as ETEC and most of them were designated as group D by phylogenetic classification. The phylogenetic analysis could be employed in veterinary laboratories in the E. coli isolates screening, including the possibility of vaccine strain selection and epidemiological searches.

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