Serogroups and virulence genes of Escherichia coli isolated from psittacine birds

Pesquisa Veterinária Brasileira

Terezinha Knöbl²  André B.s Saidenberg²  Andrea M Moreno¹  Tânia A.t Gomes¹  Mônica A.m Vieira¹  Domingos S Leite¹  Jesus E Blanco¹  Antônio J.p Ferreira¹ 

[1] ,Faculdades Metropolitanas Unidas Laboratório de Epidemiologia e Doenças Infecciosas São Paulo SP ,Brazil

关键词: Psittacine birds;    Escherichia coli;    Virulence factors;    Septicemia;    Psitacídeos;    Escherichia coli;    fatores de virulência;    septicemia;   

DOI  :  10.1590/S0100-736X2011001000013

来源: SciELO

【 摘 要 】

Escherichia coli isolates from 24 sick psittacine birds were serogrouped and investigated for the presence of genes encoding the following virulence factors: attaching and effacing (eae), enteropathogenic E. coli EAF plasmid (EAF), pili associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin (afa), capsule K1 (neu), curli (crl, csgA), temperature-sensitive hemagglutinin (tsh), enteroaggregative heat-stable enterotoxin-1 (astA), heat-stable enterotoxin -1 heat labile (LT) and heat stable (STa and STb) enterotoxins, Shiga-like toxins (stx1 and stx2), cytotoxic necrotizing factor 1 (cnf1), haemolysin (hly), aerobactin production (iuc) and serum resistance (iss). The results showed that the isolates belonged to 12 serogroups: O7; O15; O21; O23; O54; O64; O76; O84; O88; O128; O152 and O166. The virulence genes found were: crl in all isolates, pap in 10 isolates, iss in seven isolates, csgA in five isolates, iuc and tsh in three isolates and eae in two isolates. The combination of virulence genes revealed 11 different genotypic patterns. All strains were negative for genes encoding for EAF, EAEC, K1, sfa, afa, hly, cnf, LT, STa, STb, stx1 and stx2. Our findings showed that some E. coli isolated from psittacine birds present the same virulence factors as avian pathogenic E. coli (APEC), uropathogenic E. coli (UPEC) and Enteropathogenic E. coli (EPEC) pathotypes.

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