Hydrocortisone decreases apoptosis in jejunum of horses subjected to experimental ischemia and reperfusion

Pesquisa Veterinária Brasileira

Geraldo Eleno S. Alves²  Heloisa M.f. Mendes²  Tiago G.s. Alves²  Rafael R. Faleiros²  Anilton C. Vasconcelos¹  Renato L. Santos²  Jorge J.m. Rio Tinto¹ 

[1] ,Universidade Federal de Minas Gerais Escola de Veterinária Departamento de Clínica e Cirurgia VeterináriasBelo Horizonte MG ,Brazil

关键词: Horse;    ischemia;    reperfusion;    glucocorticoid;    apoptosis;    Equinos;    isquemia;    reperfusão;    glicocorticóides;    apoptose;   

DOI  :  10.1590/S0100-736X2011000600002

来源: SciELO

【 摘 要 】

In order to evaluate the effect of hydrocortisone on apoptosis in the jejunum of horses subjected to ischemia and reperfusion, ten horses were paired and grouped into two groups - treated (n=5) and non treated (n=5). Segments of the jejunum were used as controls (C), or as venous ischemia (VIsc), which were subjected to 2h of ischemia followed by 2 or 12h of reperfusion. C samples were collected at time zero (prior to ischemia) and VIsc samples were collected at 2h of ischemia and at 2 and 12h of reperfusion. TUNEL positive apoptotic cells were counted in 10 microscopical fields in deep mucosa from each horse throughout the time course. After 12h of reperfusion, the number of apoptotic cells in treated group were significantly lower than in untreated animals, indicating that hydrocortisone inhibits apoptosis. These results indicate that hydrocortisone has a beneficial effects favoring the maintenance of jejunal integrity in horses with ischemia and reperfusion injuries by preventing apoptotic cell death.

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