Morphological features and vascularization study of caprine cyclic corpus luteum

Pesquisa Veterinária Brasileira

Maria Tereza M. Miranda-moura²  Vanessa Uemura Da Fonseca¹  Naisandra Bezerra Da Silva²  Maria De Lourdes Freitas²  Osiel Benedito De Almeida²  Hugo Alexandre De Oliveira Rocha¹  Paula De Carvalho Papa¹  Carlos Eduardo Bezerra De Moura² 

[1] ,Universidade Federal do Rio Grande do Norte Centro de Biociências Departamento de MorfologiaNatal RN ,Brazil

关键词: Corpus luteum;    goat;    anatomy;    vascular density;    progesterone;    Corpo lúteo;    caprino;    anatomia;    densidade vascular;    progesterona;   

DOI  :  10.1590/S0100-736X2010000400012

来源: SciELO

【 摘 要 】

Corpus luteum is a temporary endocrine gland that regulates either the estrous cycle and pregnancy. It presents extreme dependency on the adequate blood supply. This work aims to evaluate goat corpus luteum (CL) vascular density (VD) over the estrous cycle. For that purpose, 20 females were submitted to estrus synchronization/ovulation treatment using a medroxyprogesterone intra-vaginal sponge as well as intramuscular (IM) application of cloprostenol and equine chorionic gonadotrophine (eCG). After sponge removal, estrus was identified at about 72hs. Once treatment was over, female goats were then subdivided into 4 groups (n=5 each) and slaughtered on days 2, 12, 16 and 22 after ovulation (p.o). Ovaries were collected, withdrawn and weighted. CL and ovaries had size and area recorded. Blood samples were collected and the plasma progesterone (P4) was measured through RIA commercial kits. The VD was 24.42±6.66, 36.26±5.61, 8.59±2.2 and 3.97±1.12 vessels/mm² for days 2, 12, 16 and 22 p.o, respectively. Progesterone plasma concentrations were 0.49±0.08, 2.63±0.66, 0.61±0.14 and 0.22±0.04ng/ml for days 2, 12, 16 e 22 p.o, respectively. Studied parameters were affected by the estrous cycle phase. Values greater than 12 p.o were observed. In the present work we observed that ovulation occurred predominantly in the right ovary (70% of the animals), which in turn presented bigger measures than the contra lateral one. There is a meaningful relationship between the weight and size of the ovary and these of CL (r=0.87, r=0.70, respectively, p<0.05). It is possible to conclude that morphology of goat's ovaries and plasma progesterone concentration changed according to estrous cycle stages. We propose these parameters can be used as indicators of CL functional activity.

【 授权许可】

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