Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis in Rio de Janeiro

Pesquisa Veterinária Brasileira

Shana M.o. Coelho²  Elina Reinoso¹  Ingrid A. Pereira²  Lidiane C. Soares²  Mirta Demo¹  Cristina Bogni¹  Miliane M.s. Souza¹ 

[1] ,UFRRJ

关键词: Staphylococcus aureus;    antimicrobial resistance;    virulence factors;    Staphylococcus aureus;    resistência antimicrobiana;    fatores de virulência;   

DOI  :  10.1590/S0100-736X2009000500002

来源: SciELO

【 摘 要 】

The study was conducted to characterize pheno-genotypically the virulence factors and resistance pattern of Staphylococcus aureus isolates from milk samples of cows with subclinical mastitis. All hemolytic isolates presented beta-hemolysin, and 38% of the non-hemolytic isolates were able to express hemolysins in the presence of a beta-hemolytic strain. The amplification of the coa-gene displayed four different size polymorphisms with about 400 bp, 600 bp, 700 bp and 900 bp. The spaA gene that encodes the IgG-binding region of protein A revealed sizes of 700 bp and 900 bp. The amplification of region X from spaA yielded a single amplicon for each isolate with the prevalent amplicon size being of 180 bp. Amplification of sae gene yielded an amplicon size of 920 bp in 71% of the isolates. Antibiotic resistance pattern revealed that 42% S. aureus were susceptible to all antimicrobials tested. Seven different antibiotic patterns were observed. Our results indicated that 47% and 25% of S. aureus strains exhibited resistance to penicillin and oxacillin respectively. All oxacillin-resistant isolates were mecA-positive.

【 授权许可】

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