Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever

Pesquisa Veterinária Brasileira

Julio Cesar Muñoz Paredes²  Liliane Guimarães Oliveira²  Alexandre De Carvalho Braga²  Iara Maria Trevisol¹  Paulo Michel Roehe² 

[1] ,Fundação Estadual de Pesquisa AgropecuáriaPorto Alegre RS

关键词: Classical swine fever;    classical swine fever virus;    ELISA;    serology;    Peste suína clássica;    vírus da peste suína clássica;    ELISA;    sorologia;   

DOI  :  10.1590/S0100-736X1999000300006

来源: SciELO

【 摘 要 】

An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.

【 授权许可】

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