Preliminary study of coconut water for graft tissues preservation in transplantation

Revista do Colégio Brasileiro de Cirurgiões

Jorge Miguel Schettino César¹  Andy Petroianu¹  Leonardo De Souza Vasconcelos¹  Valbert Nascimento Cardoso¹  Luciene Das Graças Mota¹  Alfredo José Afonso Barbosa¹  Cristina Duarte Vianna Soares¹  Amanda Lima De Oliveira¹ 

关键词: Coconut;    Organ preservation solutions;    Autogenous transplantation;    Ovary;    Spleen;    Skin;    Cocos;    Soluções para preservação de órgãos;    Transplante autógeno;    Baço;    Pele;    Ovário;   

DOI  :  10.1590/0100-69912015001009

来源: SciELO

【 摘 要 】

OBJECTIVE: to verify the effectiveness of coconut water in preserving tissues for transplant. METHODS: Fifty male Wistar rats were randomly distributed in five groups, according to the following preservation solutions for tissue grafts: Group 1: Lactated Ringer; Group 2: Belzer solution; Group 3: mature coconut water; Group 4: green coconut water; Group 5: modified coconut water. In Group 5, the green coconut water has been modified like the Belzer solution. From each animal we harvasted the spleen, ovaries and skin of the back segment. These tissues were preserved for six hours in one of the solutions. Then, the grafts were reimplanted. The recovery of the function of the implanted tissues was assessed 90 days after surgery, by splenic scintigraphy and blood exame. The implanted tissues were collected for histopathological examination. RESULTS: The serum levels did not differ among groups, except for the animals in Group 5, which showed higher levels of IgG than Group 1, and differences in relation to FSH between groups 1 and 2 (p <0.001), 4 and 2 (p = 0.03) and 5 and 2 (p = 0.01). The splenic scintigraphy was not different between groups. The ovarian tissue was better preserved in mature coconut water (p <0.007). CONCLUSION: the coconut water-based solutions preserves spleen, ovary, and rat skin for six hours, maintaining their normal function.

【 授权许可】

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