Revista Árvore | |
Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn | |
Vanessa Cristina Stein2  Renato Paiva1  Daiane Peixoto Vargas1  Fernanda Pereira Soares1  Eduardo Alves1  Gabriela Ferreira Nogueira1  | |
[1] ,Universidade Federal de Goiás,Brasil | |
关键词: Anthers; Ovaries; Nodal segments; Anteras; Ovários; Segmentos foliares; | |
DOI : 10.1590/S0100-67622010000500004 | |
来源: SciELO | |
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【 摘 要 】
Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis.
【 授权许可】
CC BY
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