Phylogenetic analysis of Tomato mosaic virus from Hemerocallis sp. and Impatiens hawkeri

Summa Phytopathologica

Lígia Maria Lembo Duarte²  Maria Amélia Vaz Alexandre²  Eliana Borges Rivas²  Marina Blanco Cattai¹  Rodrigo Martins Soares¹  Ricardo Harakava²  Flora Maria Campos Fernandes¹ 

[1] ,Instituto Biológico Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal São Paulo SP

关键词: maximum parsimony;    maximum likelihood;    Tobamovirus subgroups;    ornamental plants;    máxima parcimônia;    máxima verossimilhança;    subgrupos de Tobamovirus;    plantas ornamentais;   

DOI  :  10.1590/S0100-54052007000400016

来源: SciELO

【 摘 要 】

The culture and commercialization of ornamental plants have considerably increased in the last years. To supply the commercial demand, several Hemerocallis and Impatiens varieties have been bred for appreciated qualities such as flowers with a diversity of shapes and colors. With the aim of characterizing the tobamovirus isolated from Hemerocallis sp. (tobamo-H) and Impatiens hawkeri (tobamo-I) from the USA and São Paulo, respectively, as well as to establish phylogenetic relationships between them and other Tobamovirus species, the viruses were submitted to RNA extraction, RT-PCR amplification, coat-protein gene sequencing and phylogenetic analyses. Comparison of tobamovirus homologous sequences yielded values superior to 98.5% of identity with Tomato mosaic virus (ToMV) isolates at the nucleotide level. In relation to tobamo-H, 100% of identity with ToMV from tomatoes from Australia and Peru was found. Based on maximum likelihood (ML) analysis it was suggested that tobamo-H and tobamo-I share a common ancestor with ToMV, Tobacco mosaic virus, Odontoglossum ringspot virus and Pepper mild mottle virus. The tree topology reconstructed under ML methodology shows a monophyletic group, supported by 100% of bootstrap, consisting of various ToMV isolates from different hosts, including some ornamentals, from different geographical locations. The results indicate that Hemerocallis sp. and I. hawkeri are infected by ToMV. This is the first report of the occurrence of this virus in ornamental species in Brazil.

【 授权许可】

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