Desenvolvimento de um método para a determinação de tioconazol associado a nanocápsulas poliméricas por cromatografia líquida

Química Nova

Andréia P. G. Härter¹  Mariana H. Motta¹  Júlia G. De Barros¹  Roseane F. Ribeiro¹  Andréa I. H. Adams¹  Scheila R. Schaffazick¹  Cristiane De B. Da Silva¹ 

[1] ,Universidade Federal de Santa Maria Centro de Ciências da Saúde Departamento de Farmácia IndustrialSanta Maria RS ,Brasil

关键词: polymeric nanocapsules;    tioconazole;    validation;   

DOI  :  10.5935/0100-4042.20140153

来源: SciELO

【 摘 要 】

The aim of this work was to develop and validate an analytical method for the quantification of tioconazole in polymeric nanocapsule suspensions by high performance liquid chromatography with UV detection. The analysis was performed with a mobile phase composed of methanol:water (80:20) and 0.18% ammonium hydroxide; RP-18 column and UV detection at 219 nm. The method proved to be linear in the concentration range of 5-50 µg mL-1 (r = 0.9999), specific, precise (repeatability RSD = 1.42%, intermediate precision RSD = 1.17%), accurate (98 - 102%) and robust (RSD < 2.0%). In conclusion, a simple and rapid method was validated proving suitable for quantification of tioconazole in polymeric nanocapsules.

【 授权许可】

CC BY-NC   
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