Nanopartículas de poli-hidroxibutirato-co-valerato como suporte para a imobilização da lipase de Candida antarctica fração B

Química Nova

Ilizandra A. Fernandes²  Nádia L. D. Nyari²  José Vladimir De Oliveira¹  Elisandra Rigo¹  Maria Cristiane M. De Souza¹  Luciana R. B. Gonçalves¹  Sibele Berenice C. Pergher¹  Débora De Oliveira¹ 

[1] ,Universidade Regional Integrada Departamento de Ciências Agrárias Erechim RS ,Brasi

关键词: nanoparticles;    immobilization;    lipases;   

DOI  :  10.5935/0100-4042.20140055

来源: SciELO

【 摘 要 】

This work evaluates the immobilization of Candida antarctica lipase (Fraction B) using poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanoparticles as support. The effects of immobilization time (30-150 min) and pH (5-10) on lipase loading were evaluated. The stability of the immobilized enzyme towards temperature (40, 60, and 80 ºC), reuse and storage (at 4 ºC) were also determined. Furthermore, to assess its potential application in a system of interest, the immobilized lipase was used as a catalyst in the esterification of geraniol with oleic acid. The results indicated a time of 120 minutes and pH of 7 as optimal for immobilization. A 21 hour exposure of the PHBV-lipase derivative to 60 ºC showed a 33% reduction of the initial activity while storage at 4 ºC led to a residual activity (5% of the original activity). The derivative was used without significant loss of activity for 4 successive cycles. The use of the immobilized lipase as a catalyst in the production of geranyl oleate led to about 88% conversion of the initial reactants to products.

【 授权许可】

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