Electron paramagnetic resonance (EPR) spectral components of spin-labeled lipids in saturated phospholipid bilayers: effect of cholesterol

Química Nova

Heverton Silva Camargos¹  Antonio Alonso¹ 

[1] ,Universidade Federal de Goiás Instituto de Física Goiânia GO ,Brasil

关键词: EPR;    spin label;    lipid dynamics;   

DOI  :  10.1590/S0100-40422013000600013

来源: SciELO

【 摘 要 】

Electron paramagnetic resonance (EPR) spectroscopy was used to study the main structural accommodations of spin labels in bilayers of saturated phosphatidylcholines with acyl chain lengths ranging from 16 to 22 carbon atoms. EPR spectra allowed the identification of two distinct spectral components in thermodynamic equilibrium at temperatures below and above the main phase transition. An accurate analysis of EPR spectra, using two fitting programs, enabled determination of the thermodynamic profile for these major probe accommodations. Focusing the analysis on two-component EPR spectra of a spin-labeled lipid, the influence of 40 mol % cholesterol in DPPC was studied.

【 授权许可】

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