Validation of a spectrophotometric method to estimate the adsorption on nanoemulsions of an antimalarial oligonucleotide

Química Nova

Fernanda Bruxel¹  Silvia S Guterres¹  Helder F Teixeira¹ 

[1] ,Universidade Federal do Rio Grande do Sul Faculdade de Farmácia Porto Alegre RS ,Brasil

关键词: antimalarial oligonucleotides;    nanoemulsions;    UV analysis;   

DOI  :  10.1590/S0100-40422011000900026

来源: SciELO

【 摘 要 】

This study describes the validation of a spectrophotometric method to estimate oligonucleotides association with cationic nanoemulsions. Phosphodiester and phosphorothioate oligonucleotides targeting Plasmodium falciparum topoisomerase II were analyzed at 262 nm. Linear response (r > 0.998) was observed from 0.4 to 1.0 nmol/mL, the relative standard deviation values for the intra- and inter-days precision were lower than 2.6% and the recovery ranged from 98.8 to 103.6% for both oligonucleotides. The association efficiency was estimated based on an ultrafiltration/centrifugation method. Oligonucleotides recovery through 30 kDa-membranes was higher than 92%. The extent of oligonucleotides association (42 to 98%) varied with the composition of nanoemulsions

【 授权许可】

CC BY-NC   
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