Development of a flotation-spectrophotometric method for determination of cetylpyridinium chloride in pharmaceutical products

Química Nova

Hooshang Parham¹  Nahid Pourreza And Darioshi Moradi¹ 

[1] ,Shahid Chamran University Faculty of Science Department of ChemistryAhvaz,Iran

关键词: flotation-spectrophotometry;    cetylpyridinium chloride;    Orange II;   

DOI  :  10.1590/S0100-40422011000500027

来源: SciELO

【 摘 要 】

A new simple and sensitive flotation-spectrophotometric method for the determination of cetylpyridinium chloride (CPC) is reported. The method is based on the formation of an ion- associate between CPC and Orange II (OR) which is floated in the interface of aqueous phase and n-hexane by vigorous shaking. The aqueous solution was discarded and the adsorbed ion associate on to the wall of a separating funnel was dissolved in a small volume of methanol solvent and its absorbance was measured at 480 nm. The apparent molar absorptivity (Ε) of the ion associate was determined to be 4.12 x 10(5) L mol-1 cm-1. The calibration graph was linear in the concentration range of 15-800 ng mL-1 of CPC with a correlation coefficient of 0.9988. The limit of detection (LOD) was 10.8 ng mL-1. The relative standard deviation (RSD) for determination of 100 and 800 ng mL-1 of CPC was 3.47 and 2.04% (n=7), respectively. The method was successfully applied to the determination of CPC in a commercial mouth washer product.

【 授权许可】

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