Validação e estimativa da incerteza de método para análise de licopeno e β-caroteno em polpa de tomate por cromatografia líquida de alta eficiência

Química Nova

Adriana Barreto Alves²  Marta Gomes Da Silva²  Paulo Roberto Nogueira Carvalho²  Lizziane Cynara Vissotto²  Neura Bragagnolo¹ 

[1] ,Instituto de Tecnologia de Alimentos Centro de Ciência e Qualidade de Alimentos Campinas SP ,Brasil

关键词: tomato pulp;    validation;    uncertainty estimation;   

DOI  :  10.1590/S0100-40422010000900025

来源: SciELO

【 摘 要 】

A method to quantify lycopene and β-carotene in freeze dried tomato pulp by high performance liquid chromatography (HLPC) was validated according to the criteria of selectivity, sensitivity, precision and accuracy, and uncertainty estimation of measurement was determined with data obtained in the validation. The validated method presented is selective in terms of analysis, and it had a good precision and accuracy. Detection limit for lycopene and β-carotene was 4.2 and 0.23 mg 100 g-1, respectively. The estimation of expanded uncertainty (K = 2) for lycopene was 104 ± 21 mg 100 g-1 and for β-carotene was 6.4 ± 1.5 mg 100 g-1.

【 授权许可】

CC BY-NC   
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