Optimization of methodology to analyze ascorbic and dehydroascorbic acid in vegetables

Química Nova

Flávia Milagres Campos²  Sônia Machado Rocha Ribeiro²  Ceres M. Della Lucia²  Helena Maria Pinheiro-sant'ana²  Paulo César Stringheta¹ 

[1] ,Universidade Federal de Viçosa Departamento de Nutrição e Saúde

关键词: vitamin C;    extraction;    chromatography;   

DOI  :  10.1590/S0100-40422009000100017

来源: SciELO

【 摘 要 】

In this study, different solutions to extract vitamin C were tested. High-performance liquid chromatography was chosen and the conditions were based on isocratic elution in reverse phase column. Dehydroascorbic acid was determined indirectly after its reduction using dithiothreitol. The use of metaphosphoric acid to stabilize the vitamin C was shown to be required and it was necessary to neutralize the pH of the extract to apply dithiothreitol. The average recovery was 90% in collard and tomato samples. The presence of oil did not interfere in extraction and the methodology can be used to analyze stir fried vegetables.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License