Induction of transgenic hairy roots in soybean genotypes by Agrobacterium rhizogenes‑mediated transformation

Pesquisa Agropecuária Brasileira

Ricardo Luís Mayer Weber¹  Maria Helena Bodanese‑zanettini¹ 

[1] ,Universidade Federal do Rio Grande do Sul Departamento de Genética Porto Alegre RS ,Brazil

关键词: Glycine max;    composite plants;    genetic transformation;    hairy roots culture;    strain K599;    Glycine max;    plantas compostas;    transformação genética;    cultura de raízes fasciculadas;    linhagem K599;   

DOI  :  10.1590/S0100-204X2011000900014

来源: SciELO

【 摘 要 】

The objective of this work was to perform the screening of soybean genotypes as to their ability to respond to the induction of hairy roots by Agrobacterium rhizogenes‑mediated transformation. Four Brazilian soybean cultivars (BRSMG 68 Vencedora, BRS 137, Embrapa 48, and MG/BR 46 Conquista) and two North American ones adapted to Brazilian cropping conditions (Bragg and IAS‑5) were screened for their capacity to respond to A. rhizogenes in protocols for in vitro hairy root culture and ex vitro composite plant production. Four‑day‑old seedlings with uniform size were injected with A. rhizogenes harboring the plasmid p35S‑GFP. Seedlings expressing green fluorescent protein (GFP) in at least one hairy root were used to determine the transformation frequency. Using an axenic in vitro protocol, excised cotyledons from four‑day‑old seedlings were infected with A. rhizogenes harboring the pCAMBIA1301 plasmid, containing the gusA reporter gene. The transformation frequency and the number of days for hairy root emergence after bacterial infection (DAI) were evaluated. The transformation frequency and DAI varied according to the genotype. Cultivars MG/BR 46 Conquista and BRSMG 68 Vencedora are more susceptible to A. rhizogenes and can be recommended for transformation experiments.

【 授权许可】

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