In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

Pesquisa Agropecuária Brasileira

Tatiane Loureiro Da Silva²  Jonny Everson Scherwinski-pereira¹ 

[1] ,Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Piracicaba SP ,Brazil

关键词: ex situ conservation;    germplasm bank;    micropropagation;    conservação ex situ;    banco do germoplasma;    micropropagação;   

DOI  :  10.1590/S0100-204X2011000400007

来源: SciELO

【 摘 要 】

The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License