Potential of antimicrobial volatile organic compounds to control Sclerotinia sclerotiorum in bean seeds

Pesquisa Agropecuária Brasileira

Maurício Batista Fialho¹  Maria Heloisa Duarte De Moraes¹  Annelise Roberta Tremocoldi¹  Sérgio Florentino Pascholati¹ 

[1] ,Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de Fitopatologia e NematologiaPiracicaba SP ,Brazil

关键词: Phaseolus vulgaris;    alternative control;    fumigation;    white mold;    yeast;    Phaseolus vulgaris;    controle alternativo;    fumigação;    mofo-branco;    levedura;   

DOI  :  10.1590/S0100-204X2011000200004

来源: SciELO

【 摘 要 】

The objective of this work was to evaluate the potential of an artificial mixture of volatile organic compounds (VOCs), produced by Saccharomyces cerevisiae, to control Sclerotinia sclerotiorum in vitro and in bean seeds. The phytopathogenic fungus was exposed, in polystyrene plates, to an artificial atmosphere containing a mixture of six VOCs formed by alcohols (ethanol, 3-methyl-1-butanol, 2-methyl-1-butanol and phenylethyl alcohol) and esters (ethyl acetate and ethyl octanoate), in the proportions found in the atmosphere naturally produced by yeast. Bean seeds artificially contamined with the pathogen were fumigated with the mixture of VOCs in sealed glass flasks for four and seven days. In the in vitro assays, the compounds 2-methyl-1-butanol and 3-methyl-1-butanol were the most active against S. sclerotiorum, completely inhibiting its mycelial growth at 0.8 µL mL-1, followed by the ethyl acetate, at 1.2 µL mL-1. Bean seeds fumigated with the VOCs at 3.5 µL mL-1 showed a 75% reduction in S. sclerotiorum incidence after four days of fumigation. The VOCs produced by S. cerevisiae have potential to control the pathogen in stored seeds.

【 授权许可】

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