Phytogeographical origin of Madeiran common beans based on phaseolin patterns

Pesquisa Agropecuária Brasileira

Emanuel Marques Da Silva²  Anísia Soraia Abreu Correia²  Nuno Alexandre Amaral Lopes²  Humberto Gil Moreira Nóbrega²  José Filipe Teixeira Ganança²  Ana Maria Domingues¹  Manhaz Khadem²  Jan Jacek Slaski¹  Miguel Ângelo Almeida Pinheiro De Carvalho² 

[1] ,Universidade da Madeira Centro de Estudos da Macaronésia ISOPlexis Banco de GermoplasmaFunchal,Portugal

关键词: Phaseolus vulgaris;    gene pool;    genetic variability;    germplasm;    lab-on-a-chip technology;    Phaseolus vulgaris;    pool genético;    variabilidade genética;    germoplasma;    tecnologia lab-on-a-chip;   

DOI  :  10.1590/S0100-204X2010000800012

来源: SciELO

【 摘 要 】

The objective of this work was to determine the geographic origin of the Madeiran common bean (Phaseolus vulgaris) gene pool. Phaseolin patterns of 50 accessions representing the diversity of common bean collected in Madeira, Portugal, and conserved in the ISOPlexis Germplasm Bank, were analysed using the Experion automated electrophoresis system, based on lab-on-a-chip technology. Five common bean standard varieties with typical phaseolin patterns were used to determine the phytogeographical origin of the Madeiran common bean accessions. Ninety two percent of the accessions exhibited a phaseolin pattern consistent with the one of common bean types belonging to the Andean gene pool, while the origin of the remaining 8% of the accessions was indistinguishable. The application of a similarity coefficient of 85%, based on Pearson correlations, increases the number of accessions with uncertain pattern. The analytical approach used permitted the determination of the origin of the common bean gene pool, which is Andean in 98% of the cases, and clustering of the observed variability among the Madeiran common beans.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License