Screening of Brazilian soybean genotypes with high potential for somatic embryogenesis and plant regeneration

Pesquisa Agropecuária Brasileira

Annette Droste²  Anelise Machado Da Silva¹  Iliane Freitas De Souza¹  Beatriz Wiebke-strohm¹  Lauro Bücker-neto¹  Marta Bencke¹  Márcia Viviane Sauner¹  Maria Helena Bodanese-zanettini¹ 

[1] ,Universidade FeevaleNovo Hamburgo RS ,Brazil

关键词: Glycine max;    Brazilian cultivars;    embryogenic potential;    genetic engineering;    soybean transformation;    Glycine max;    cultivares brasileiras;    potencial embriogênico;    engenharia genética;    transformação de soja;   

DOI  :  10.1590/S0100-204X2010000700012

来源: SciELO

【 摘 要 】

The aim of this work was to identify Brazilian soybean (Glycine max) genotypes with potential to respond to in vitro culture stimuli for primary somatic embryo induction, secondary embryo proliferation and plant regeneration. Differences among eight tested cultivars were observed at each stage. Two cultivars, IAS-5 and BRSMG 68 Vencedora, were selected for the evaluation of the capacity for embryo differentiation and plant regeneration. These cultivars had high embryo induction frequencies, repetitive embryogenic proliferation, and low precocious embryo germination in the initial experiment. The effect of abscisic acid (ABA) and charcoal addition on plant regeneration was investigated. The addition of ABA to proliferation medium and of ABA and activated charcoal to maturation medium increased embryo differentiation rates, which resulted in a higher number of regenerated plants. The BRSMG 68 Vencedora cultivar was found to have a high potential for embryo induction, embryo proliferation and plant regeneration. The potential of this cultivar for somatic embryogenesis was similar to that observed for cultivar IAS-5, which is currently used for soybean transformation in Brazil. BRSMG 68 Vencedora may be a good alternative genotype for soybean genetic engineering via somatic embryogenesis protocols.

【 授权许可】

CC BY   
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