Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans

Pesquisa Agropecuária Brasileira

Patrícia Valle Pinheiro²  Josias Corrêa De Faria²  Elsa Oliveira Paranaguá E Lago Nogueira¹  Francisco José Lima Aragão¹ 

[1] ,Embrapa Arroz e FeijãoSanto Antônio de Goiás GO

关键词: Phaseolus;    BCMNV;    genetic engineering;    genetic variability;    transgene genotypic stability;    Phaseolus;    BCMNV;    engenharia genética;    variabilidade genética;    estabilidade genotípica do transgene;   

DOI  :  10.1590/S0100-204X2009000900015

来源: SciELO

【 摘 要 】

The objective of the present work was to determine the inheritance and stability of transgenes of a transgenic bean line expressing the genes rep-trap-ren from Bean golden mosaic virus and the bar gene. Crosses were done between the transgenic line and four commercial bean cultivars, followed by four backcrosses to the commercial cultivars. Progenies from each cross were evaluated for the presence of the transgenes by brushing the leaves with glufosinate ammonium and by polymerase chain reaction using specific oligonucleotides. Advanced generations were rub-inoculated with an isolate of Bean common mosaic necrosis virus (BCMNV). The transgenes were inherited consistently in a Mendelian pattern in the four crosses studied. The analyzed lines recovered close to 80% of the characteristics of the recurrent parent, as determined by the random amplified DNA markers used, besides maintaining important traits such as resistance to BCMNV. The presence of the transgene did not cause any detectable undesirable effect in the evaluated progenies.

【 授权许可】

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