Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

Pesquisa Agropecuária Brasileira

Lucymeire Souza Morais-lino¹  Janay Almeida Dos Santos-serejo²  Sebastião De Oliveira E Silva²  José Raniere Ferreira De Santana¹  Adilson Kenji Kobayashi² 

[1] ,Universidade Estadual de Feira de SantanaFeira de Santana BA ,Brazil

关键词: Musa;    culture media;    propagation;    somatic embryogenesis;    Musa;    meio de cultura;    micropropagação;    embriogênese somática;   

DOI  :  10.1590/S0100-204X2008001000010

来源: SciELO

【 摘 要 】

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

【 授权许可】

CC BY   
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