Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles

Pesquisa Agropecuária Brasileira

Enderson Petrônio De Brito Ferreira²  André Nepomuceno Dusi¹  Gustavo Ribeiro Xavier¹  Norma Gouvêa Rumjanek¹ 

[1] ,Embrapa Arroz e FeijãoSanto Antônio de Goiás GO ,Brazil

关键词: Solanum tuberosum;    associative bacteria;    molecular marker;    PCA;    Solanum tuberosum;    bactérias associativas;    marcador molecular;    PCA;   

DOI  :  10.1590/S0100-204X2008000500008

来源: SciELO

【 摘 要 】

The objective of this work was to determine the shifts on the PCR-DGGE profiles of bacterial communities associated to the rhizosphere of potato cultivars, in order to generate baseline information for further studies of environmental risk assessment of genetically modified potato plants. A greenhouse experiment was carried out with five potato cultivars (Achat, Bintje, Agata, Monalisa and Asterix), cultivated in pots containing soil from an integrated system for agroecological production. The experiment was conducted in a split plot randomized block design with five cultivars, three sampling periods and five replicates. Rhizosphere samples were collected in three sampling dates during plant development. DNA of rhizosphere microorganisms was extracted, amplified by PCR using bacterial universal primers, and analyzed through DGGE. Shifts on the rhizosphere bacterial communities associated to rhizosphere of different cultivars were related to both cultivar and plant age. Differences among rhizosphere bacterial communities were clearest at the earliest plant age, tending to decrease in later stages. This variation was detected among bacterial communities of the five tested cultivars. The characterization of soil microbial communities can be part of plant breeding programs to be used on studies of environmental risk assessment of genetically modified potatoes.

【 授权许可】

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