Detection of siderophores in endophytic bacteria Methylobacterium spp. associated with Xylella fastidiosa subsp. pauca

Pesquisa Agropecuária Brasileira

Paulo Teixeira Lacava²  Maria Estela Silva-stenico¹  Welington Luiz Araújo²  Ana Valéria Colnaghi Simionato¹  Emanuel Carrilho¹  Siu Mui Tsai¹  João Lúcio Azevedo² 

[1] ,Escola Superior de Agricultura Luiz de Queiroz Departamento de Genética Piracicaba SP ,Brazil

关键词: citrus variegated chlorosis;    endophytes;    heterologous siderophores;    iron;    symbionts;    clorose variegada dos citros;    endófitos;    sideróforos heterólogos;    ferro;    simbiose;   

DOI  :  10.1590/S0100-204X2008000400011

来源: SciELO

【 摘 要 】

The objective of this work was to study the production of siderophores by endophytic bacteria Methylobacterium spp., which occupy the same ecological niche as Xylella fastidiosa subsp. pauca (Xfp) in citrus plants. The siderophore production of Methylobacterium strains was tested according to chromeazurol agar assay test (CAS), Csáky test (hydroxamate-type) and Arnow test (catechol-type). In addition, the ability of Xfp to use siderophores, in vitro, produced by endophytic bacteria as source of iron, was evaluated. All 37 strains of Methylobacterium spp. tested were CAS-positive for siderophore production. Methylobacterium spp. produced hydroxamate-type, but not catechol-type siderophores. In vitro growth of Xfp was stimulated by the presence of supernatant siderophores of endophytic Methylobacterium mesophilicum.

【 授权许可】

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