Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines

Pesquisa Agropecuária Brasileira

Caroline Pereira Petrillo²  Newton Portilho Carneiro¹  Antônio Álvaro Corsetti Purcino¹  Carlos Henrique Siqueira Carvalho¹  José Donizeti Alves²  Andréa Almeida Carneiro¹ 

[1] ,Universidade Federal de Lavras Departamento de Fisiologia Vegetal Lavras MG ,Brazil

关键词: Zea mays;    ammonium glufosinate;    beta-glucuronidase;    immature embryos;    phosphinotricin acetyl transferase;    plant transformation;    Zea mays;    glufosinato de amônio;    beta-glucuronidase;    embriões imaturos;    fosfinotricina acetil transferase;    transformação de plantas;   

DOI  :  10.1590/S0100-204X2008000300012

来源: SciELO

【 摘 要 】

The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics.

【 授权许可】

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