Enzyme activities and pectin breakdown of sapodilla submitted to 1-methylcyclopropene

Pesquisa Agropecuária Brasileira

Patrícia Lígia Dantas De Morais²  Luiz Carlos De Oliveira Lima¹  Maria Raquel Alcântara De Miranda¹  José Donizete Alves¹  Ricardo Elesbão Alves¹  José Daniel Silva¹ 

[1] ,Universidade Federal Rural do Semi-ÁridoMossoró RN ,Brazil

关键词: Manilkara zapota;    beta-galactosidase;    ripening;    Manilkara zapota;    beta-galactosidase;    amadurecimento;   

DOI  :  10.1590/S0100-204X2008000100003

来源: SciELO

【 摘 要 】

The objective of this work was to investigate the influence of 1-methylcyclopropene (1-MCP) at 300 nL L-1 on activities of cell wall hidrolytic enzymes and pectin breakdown changes which Sapodilla (Manilkara zapota cv. Itapirema 31) cell wall undergoes during ripening. Sapodilla were treated with ethylene antagonist 1-MCP at 300 nL L-1 for 12 hours and then, stored under a modified atmosphere at 25º C for 23 days. Firmness, total and soluble pectin and cell wall enzymes were monitored during storage. 1-MCP at 300 nL L-1 for 12 hours delayed significantly softening of sapodilla for 11 days at 25º C. 1-MCP postharvest treatment affected the activities of cell wall degrading enzymes pectinmethylesterase and polygalacturonase and completely suppressed increases in beta-galactosidase for 8 days, resulting in less pectin solubilization. Beta-galactosidase seems relevant to softening of sapodilla and is probably responsible for modification of both pectin and xyloglucan-cellulose microfibril network.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License