Somatic embryogenesis and in vitro plant regeneration from pejibaye adult plant leaf primordia

Pesquisa Agropecuária Brasileira

Marcílio De Almeida¹  Cristina Vieira De Almeida¹ 

[1] ,Escola Superior de Agricultura Luiz de Queiroz Dep. de Ciências Biológicas Piracicaba SP ,Brazil

关键词: Bactris gasipaes;    histology;    micropropagation;    peach palm;    Bactris gasipaes;    histologia;    micropropagação;   

DOI  :  10.1590/S0100-204X2006000900015

来源: SciELO

【 摘 要 】

The aim of this work was to evaluate a protocol for plant regeneration by means of somatic embryos obtained from isolated adult pejibaye leaf primordia, and to describe histological origin of embryos and morphogenetic response. Explants were cultivated in modified MS medium. Mesophyll parenchymatous cells originated meristemoids (preembryonic complex formation) induced with 7.1 µM BAP in the first two subculture periods. After polarized structures with 12.9 µM NAA and 3.55 µM BAP were formed in the third subculture, somatic embryos developed and regenerated normal plants. The mesophyll parenchymatous cells display high capacity of direct response to the auxin and cytokinin.

【 授权许可】

CC BY   
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